UHPLC Column, RP Phenyl Hexyl, 1.8um, 100A. 2.1mm ID x 100mm Length. Cogent Brand. 1 EA.

Additional Information:

Cogent Phenyl Hexyl™ is a reversed-phase UHPLC stationary phase designed to provide enhanced selectivity for aromatic and moderately hydrophobic compounds. Manufactured with a phenyl ring attached through a six-carbon linker and fully double end capped on ultra-high purity silica, this phase offers strong π-π interactions while minimizing the influence of residual silanol activity. The extended C6 linker provides different selectivity compared to traditional Phenyl phases, making it especially useful for aromatic analytes, positional isomers, pharmaceutical compounds, flavonoids, tocopherols, and natural products.

68139-10P-2 is a UHPLC column packed with 1.8 µm Phenyl Hexyl™ particles featuring a 100 Å pore size in a 2.1 mm ID x 100 mm format. This popular configuration combines excellent resolution with reduced solvent consumption and is frequently selected for LC-MS applications. The 100 mm length provides greater peak capacity than shorter column formats, while the sub-2 µm particle technology delivers exceptional efficiency for demanding separations. Typical operating flow rates are approximately 0.2–0.4 mL/min. Because of the low flow rates required by 2.1 mm ID columns, standard HPLC systems must be capable of precise and reproducible low-flow operation if they are used with this column.

Designed specifically for high-pressure UHPLC applications, this column is ideal for aromatic compounds requiring enhanced selectivity and high-resolution separations. Methanol is often preferred over acetonitrile when maximizing π-π interactions is desired. While compatible HPLC systems may be used if pressure limits permit, this column is designed primarily for UHPLC operation where the full benefits of sub-2 µm particle technology can be realized.

Key Benefits

  • Phenyl Hexyl™ stationary phase

  • Fully double end capped

  • Six-carbon linker with terminal phenyl group

  • Ultra-high purity silica support

  • Enhanced π-π interaction selectivity

  • Designed for UHPLC applications

  • High-efficiency 1.8 µm particles

  • Increased peak capacity

  • Ideal for LC-MS methods

  • Reduced solvent consumption

Typical Applications

  • LC-MS methods

  • Aromatic compound analysis

  • Positional isomer separations

  • Tocopherol analysis

  • Flavonoid analysis

  • Polynuclear aromatic hydrocarbon analysis

  • Nitro-aromatic compound analysis

  • API analysis

  • Natural product analysis

  • Pharmaceutical method development

Specifications

  • Product Type: UHPLC Column

  • Stationary Phase: Phenyl Hexyl™

  • Chromatography Mode: Reversed Phase

  • Bonded Phase: Phenyl Hexyl

  • Endcapping: Fully Double End Capped

  • Particle Size: 1.8 µm

  • Pore Size: 100 Å

  • Column Dimensions: 2.1 mm ID x 100 mm

  • Length: 100 mm

  • Typical Flow Rate: 0.2–0.4 mL/min

  • Brand: Cogent

Important Notes

  • Designed specifically for UHPLC operation and high-pressure reversed-phase chromatography

  • 1.8 µm particles generate substantially higher backpressure than conventional HPLC columns

  • Maximum performance is achieved on UHPLC systems designed for sub-2 µm particle technology

  • The 2.1 mm ID format is commonly used for LC-MS applications

  • Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.4 mL/min

  • The 100 mm length provides greater resolution and peak capacity than 50 mm columns

  • Phenyl Hexyl chemistry may provide different selectivity than traditional Phenyl phases

  • Methanol may enhance π-π interactions compared to acetonitrile for certain applications

  • Excellent choice for aromatic compounds containing benzene rings

  • Samples should be filtered through a 0.2 µm syringe filter prior to injection


 

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